Composite

Part:BBa_M36014

Designed by: Jesse Palmer, Elliot Lui   Group: Stanford BIOE44 - S11   (2011-05-02)

Glycogen Synthesis Actuator (alt2)

Input: PoPs Output: Glycogen overproduction

Expresses glgC mutant gene (coding for the glucose-1-phosphate adenylyltransferase) and glgA gene (coding for glycogen synthase). These genes are expressed normally for e. coli metabolism. This part is intended to be used for over expression and overproduction of glycogen.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1996
    Illegal PstI site found at 2428
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1996
    Illegal PstI site found at 2428
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1996
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1996
    Illegal PstI site found at 2428
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 618
    Illegal EcoRI site found at 1115
    Illegal EcoRI site found at 1996
    Illegal PstI site found at 2428
    Illegal AgeI site found at 2620
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2344


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
None